Journal: Neural Regeneration Research

Article Title: A vascular endothelial growth factor–loaded chitosanhyaluronic acid hydrogel scaffold enhances the therapeutic effect of adipose-derived stem cells in the context of stroke

doi: 10.4103/nrr.nrr-d-24-00129

Figure Lengend Snippet: Figure 1 ｜ADSC culture and identity verification, and the effect of VEGF on ADSCs. (A) Representative images of ADSCs on days 3 (i) and 5 (ii) of primary culture, as well as third-passage ADSCs (iii). On day 3, the cells were spindle-shaped, isolated, scattered, and adherent. By day 5, the cells had proliferated rapidly, and the cell colonies had expanded. The morphology of the third-passage cells was uniform, displaying a characteristic fish-like, fibrous, and swirling arrangement. Scale bar: 100 μm. (B) Flow cytometric analysis of CD90, CD29, CD34, and CD45 expression on the cell surface of third-passage ADSCs. (C) CCK-8 analysis of the viability of ADSCs treated with different concentrations of VEGF. Data are expressed as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, vs. Cont group (one-way analysis of variance with Bonferroni’s post hoc test). (D) Enzyme-linked immunosorbent assay analysis of VEGF, b-FGF, TGF-β, BDNF, and IGF-1 concentration by ADSC cells treated with VEGF (50 ng/mL) or left untreated. Data are expressed as mean ± SEM (n = 12). **P < 0.01, ***P < 0.001, vs. VEGF- group (one-way analysis of variance with Bonferroni’s post hoc test [C] or unpaired t-test [D]). ADSCs: Adipose-derived mesenchymal stem cells; BDNF: brain-derived neurotrophic factor; b-FGF: basic fibroblast growth factor; Cont: control; IGF- 1: insulin-like growth factor-1; TGF-β: transforming growth factor-β; VEGF: vascular endothelial growth factor.

Article Snippet: The following fluorochrome-labeled antibodies were utilized: APC-cy7-CD45 (BD Biosciences, San Jose, CA, USA, Cat# 561586, RRID: AB_10896305), PE-CD34 (RayBiotech, Norcross, GA, USA, Cat# 188-10041-PE, RRID: AB_2315728), FITC-CD90 (BD Biosciences, Cat# 561973, RRID: AB_395588), and BV421-CD29 (BD Biosciences, Cat# 564131, RRID: AB_2738613).

Techniques: Isolation, Expressing, CCK-8 Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay, Derivative Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Effect of rapamycin on endometriosis in mice

doi: 10.3892/etm.2016.3280

Figure Lengend Snippet: Representative images of immunohistochemical analysis of CD34 in endometriotic lesions of the (A) control, (B) saline and (C) rapamycin-treated groups. Staining was performed with the streptomycin avidin-peroxidase method. Magnification, ×400.

Article Snippet: Mouse anti-human VEGF (1:200; cat. no. RB-9031) and CD34 (1:100; cat. no. MS-363) monoclonal antibodies, streptomycin avidin-peroxidase (SP) immunochemistry (cat. no. Kit-9701) and DAB (cat. no. Kit-0017) kits were from Maxim Biotech, Inc. (Fuzhou, China).

Techniques: Immunohistochemical staining, Control, Saline, Staining, Avidin-Biotin Assay

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Relative Computed Tomography (CT) Enhancement Value for the Assessment of Microvascular Architecture in Renal Cell Carcinoma

doi: 10.12659/MSM.902957

Figure Lengend Snippet: CT scan and immunohistochemical staining results (CD34, 200×) of three cases. ( A1, A2 , a 67-year-old woman): Obvious heterogeneous contrast enhancement in the right kidney (bold arrow) with a VTA value of 160 HU and a RTV value of 1.3 was displayed. There were many microvessels which mainly consisted of relatively mature vessels with lumen (bold arrow) and the microvascular areas were the largest. ( B1, B2 , a 44-year-old man): Moderate homogeneous contrast enhancement in the left kidney (curved arrow) with a VTA value of 87 HU and a RTV value of 0.75 was displayed. Microvessels mainly consisted of vessels with fissured lumen (curved arrow) and the microvascular area was large. ( C1, C2 , a 59-year-old man): Mild contrast enhancement (long arrow) with a VTA value of 80 HU and a RTC value of 0.6 was displayed. Pseudo-capsule (short arrow) appeared as hyper density in the lesion margin. There were a few microvessels which involvement in microvessels without lumen mostly (long and short arrows) and the microvascular area was the smallest.

Article Snippet: Tissue sections were stained using mouse anti-human monoclonal CD34 antibody and goat anti-mouse polyclonal antibody (Fuzhou Maxim Biotechnology Co. Ltd., Fuzhou, China) using the streptavidin-peroxidase method, following the instructions provided by the company.

Techniques: Computed Tomography, Immunohistochemical staining, Staining